DNA from Saliva Is Highly Pure, Despite Low A260/A230 Ratios
Saliva samples in the spin column-based saliva DNA purification/extraction kit contain large amounts of carbohydrates (from the highly glycosylated protein mucin). Although the protein is removed during extraction, a small amount of this carbohydrate remains. Carbohydrates absorb very strongly at 230 nm, so even small amounts of carbohydrates can increase the 230 reading, resulting in […]
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