DNA in the saliva is suitable for whole genome sequencing (WGS) with the magnetic bead-based saliva DNA extraction kit. There are many scientific references to the fruitful use of saliva in sequencing in small and large studies. DNA extracted from these archived saliva samples, which the survey sequenced, covered 97.8-98.2% of the genome.
After identifying the variants in these samples, Dr. McLean compared the results with data from the same cohort previously determined using the genotyping system and found 99.91-99.97% concordance, indicating that Oragene/saliva samples provide consistent results across technology platforms. A question that many researchers continue to ask is: What effect do salivary bacteria have on sequencing? The studies show that bacterial content does not affect variant calling during sequencing.
Magnetic Bead Based Cell Free DNA Extraction – Process of DNA Extraction
Since DNA studies the genetic cause of the disease, like the drugs, paternity tests, presence of antibodies or lack of their presence, the extraction of the said DNA is very important. What is also important is to use state-of-the-art equipment like the Magnetic Bead Based Cell-Free DNA Extraction/purification kit to extract the DNA. It also helps detect viruses and bacteria; not just that, it also helps with DNA sequencing and –forensic research.
Scientists must follow various methods and steps to extract DNA. However, DNA extraction has three basic steps: Lysis, Precipitation and Purification.
- Purification Binding
The last process is the purification matrix binding. With the Magnetic Bead Based Solution, the scientists can use cellulose, silica, and ion exchange for binding to genomic DNA. Through this process, the chemistry influences the purity and efficiency of the DNA isolation.
Binding capacity refers to the amount of nucleic acid that can bind before the system reaches its full capacity of the system and stops the binding process. The mediums can selectively bind to DNA, leaving out RNA and proteins.
